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ITEA Der f 2 ELISA Kit

ITEA Der f 2 ELISA Kit

Item # Pack Size Storage Price
1-DF2-001 96 wells/kit Store at 2-8℃ 80,000

ELISA kit for quantification of the group 2 allergen from house dust mites (Dermatophagoides farinae), Der f 2.
The pre-blocked and coated microplates with captured antibodies and
the use of enzyme-labeled detection antibodies enable easy and rapid measurement.
* Der f 2 concentration in samples including Der f 2 and Der p 2 cannot be precisely quantified with this kit which has cross-reactivity to Der f 2 and Der p 2 both, and a standard for Der f 2 were used in allergen quantification.

  • ■ Usage examples

    ・Measurement of Der 1 in house dust
    ・Measurement of Der 1 in bedding
    ・Measurement of Der 1 in foods
    ・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)

  • ■ Kit Contents

    Component Pack size
    Pre-blocked and coated microplates with captured antibodies, 6 strips of 8 wells2 packs
    Standard (lyophilized) for 2 measurements2
    Enzyme-labeled anti-Der f 2 antibody, 12 mL1
    TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution, 12 mL1
    Stop solution, 12 mL1
    Dilute solution, 30 mL2
    Washing solution (20x concentrated), 30 mL1
    Microplate seal3

    * Der f 2 concentration in samples including Der f 2 and Der p 2 cannot be precisely quantified with this kit which has cross-reactivity to Der f 2 and Der p 2 both, and a standard for Der f 2 were used in allergen quantification.

  • ■ Performance

    Measurement range 5-0.08 ng/mL
    Detection limit 0.08 ng/mL
    Curve fit 4-parameter logistic, or cubic formula recommended

    ■ Measurement accuracy

    Within-day imprecision CV <6%
    Between-day imprecision CV <6%
    Intermediate precision CV <8%

    Der f 2 sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.

  • ■ Standard curve example

    Standard curve example
  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse
    Rat
    Human
    Rabbit
    Dog
    Bovine fetus
    cell culture medium
    (10% bovine fetus serum added)
    RPMI
    DMEM

Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.