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ITEA Der f 1 ELISA Kit, High Sensitive

ITEA Der f 1 ELISA Kit, High Sensitive

Item # Pack Size Storage Price
1-DFH-001 96 wells/kit Store at 2-8℃ 80,000

This is a high sensitive ELISA kit which is available for measurement of Der f 1 at low concentration, 31.2~2000 pg/mL. It is suitable for measurement of a very small amount of Der f 1 in air or house dust, inspection for mite contamination in food ingredients, and verification of allergen reduction effect.

  • ■ Key Benefits

    ・High sensitivity: the detection limit is 31.2 pg/mL
    ・Mesurement with an absorbance plate reader (450 nm)
    ・Quick measurement: test results in 3 hours and 45 minutes
    ・High specificity: having no cross reactivity with Der p 1
    ・Can be performed at room temperature (no incubator required)
    ・Easy to use: microplates that can be used without washing after opening
    ・Compact package

  • ■ Usage examples

    ・Measurement of Der 1 in house dust
    ・Measurement of Der 1 in bedding
    ・Measurement of Der 1 in foods
    ・Development and evaluation of products against house dust mites (bedding, filters, air purifiers, liquid agents, cleaners and so on)

  • ■ Kit Contents

    Component Pack size
    Pre-blocked and coated microplates with captured antibodies, 6 strips of 8 wells2 packs
    Standard (lyophilized) for over 4 measurements1
    Biotin-labeled anti-Der f 1 antibody, 120 µL1
    Enzyme-labeled anti-Der f 1 antibody, 120 µL1
    TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution, 12 mL1
    Stop solution, 12 mL1
    Dilute solution, 30 mL2
    Washing solution (20x concentrated), 30 mL1
    Microplate seal3
  • ■ Performance

    Measurement range 2000-31.2 pg/mL
    Detection limit 31.2 pg/mL
    Curve fit 4-parameter logistic, or cubic formula recommended

    ■ Measurement accuracy

    Within-day imprecision CV < 5%
    Intermediate precision CV < 5%

    Six samples adjusted to low, medium, and high concentration ranges within the standard curve were independently measured four times and calculated.

  • ■ Standard curve example

    Standard curve example
  • ■ Specificity

    Der f 1 was specifically measured and Der p 1(0.4、4、40、400、4000 ng/mL) was no cross with in this assay.

  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse
    Rat
    Human
    Rabbit
    Dog
    Bovine fetus
    cell culture medium
    (10% bovine fetus serum added)
    RPMI
    DMEM

Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.