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ITEA Cry j 1 ELISA Kit

ITEA Cry j 1 ELISA Kit

Item # Pack Size Storage Price
1-CJ1-001 96 wells/kit Store at 2-8℃ 60,000

ELISA kit for quantification of the major allergen from Cryptomeria japonica, Cry j 1.
The pre-blocked and coated microplates with captured antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement.

  • ■ Key Benefits

    ・High sensitivity: the detection limit is 0.16 ng/mL.
    ・Quick measurement: test results in 2 hours and 15 minutes
    ・High specificity: Cry j 1 specific, having no cross reactivity with Cry j 2
    ・Can be performed at room temperature (no incubator required)
    ・Easy to use: microplates that can be used without washing after opening
    ・Compact package

  • ■ Usage examples

    ・Control of the pollen lots
    ・Measurement of Cry j 1 in house dust
    ・Measurement of Cry j 1 in the air
    ・Evaluation and control of Cry j 1 concetration in a chamber that has been scattered
    ・Development and evaluation of products against the pollen (filters, air purifiers, cleaners and so on)

  • ■ Kit Contents

    Component Pack size
    Pre-blocked and coated microplates with captured antibodies, 6 strips of 8 wells2 packs
    Standard (lyophilized) for 2 measurements2
    Enzyme-labeled anti-Cry j 1 antibody, 12 mL1
    TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution, 12 mL1
    Stop solution, 12 mL1
    Dilute solution, 30 mL2
    Washing solution (20x concentrated), 30 mL1
    Microplate seal3
  • ■ Performance

    Measurement range 10-0.16 ng/mL
    Detection limit 0.16 ng/mL
    Curve fit 4-parameter logistic, or cubic formula recommended

    ■ Measurement accuracy

    Within-day imprecision CV <3%
    Between-day imprecision CV <7%
    Intermediate precision CV <8%

    Cry j 1 sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.

  • ■ Standard curve example

    Standard curve example
  • ■ Specificity

    Cry j 1 was specifically measured and Cry j 2 (0.1、1、10、100 ng/mL) was no cross with in this assay.

  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse
    Rat
    Human
    Rabbit
    Dog
    Bovine fetus
    cell culture medium
    (10% bovine fetus serum added)
    RPMI
    DMEM

  • ■ Reference

    Terumi Midoro-Horiuti et al. “Structural basis for epitope sharing between group 1 allergens of cedar pollen.” Mol Immunol 43 (2006): 509-518.[PubMed]Sakaguchi, Masahiro et al. “Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis.” Immunology 91.2 (1997): 161-6. [PubMed]

    高橋裕一、宮澤博、阪口雅弘、井上栄、片桐進、名古屋隆生、渡辺雅尚、谷口美文、栗本雅司、安枝浩, 室内塵中のCryj1量と空中スギ花粉数との関係、アレルギー 1994; 43(2):97-100.

    渡辺雅尚、田村正広、名古屋隆生、高橋裕一、片桐進、岡鉄雄, スギ花粉抗原(CryjI)に対するモノクローナル抗体を用いたEnzyme-LinkedImmunosorbentAssay(ELISA)によるCryjIの測定、アレルギー 1992; 41(11): 1535-1539.

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Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.