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ITEA OVA ELISA Kit

ITEA OVA ELISA Kit

Item # Pack Size Storage Price
1-OVA-001 96 wells/kit Store at 2-8℃ 80,000

ELISA kit for quantification of OVA(Ovalbumin) from chickens. The pre-blocked and coated microplates with captured antibodies and the use of enzyme-labeled detection antibodies enable easy and rapid measurement. It is useful for experiments on OVA kinetics in vivo.

  • ■ Key Benefits

    ・Quick measurement: test results in 2 hours and 15 minutes
    ・Can be performed at room temperature (no incubator required)
    ・Easy to use: microplates that can be used without washing after opening
    ・Compact package

  • ■ Usage examples

    ・Quantification of OVA reagents for experiment
    ・Evaluation of transfected OVA expression
    ・Determination of OVA concentrations in blood and milk

  • ■ Kit Contents

    Component Pack size
    Pre-blocked and coated microplates with captured antibodies, 6 strips of 8 wells2 packs
    Standard (lyophilized) for 4 measurements1
    Enzyme-labeled anti-OVA antibody, 12 mL1
    TMB (3, 3', 5, 5'-tetramethylbenzidine) Solution, 12 mL1
    Stop solution, 12 mL1
    Dilute solution, 30 mL2
    Washing solution (20x concentrated), 30 mL1
    Microplate seal3
  • ■ Performance

    Measurement range 50-0.78 ng/mL
    Detection limit 0.78 ng/mL
    Curve fit 4-parameter logistic, or cubic formula recommended

    ■ Measurement accuracy

    Within-day imprecision CV <3%
    Between-day imprecision CV <14%
    Intermediate precision CV <14%

    OVA sample solutions were measured in triplicate x 6 times and the reproducibilities were evaluated.

  • ■ Standard curve example

    Standard curve example
  • ■ Spike and recovery test

    Sample Dilution ratio Recovery rate(%)
    Normal serum Mouse 1:10 96.5
    Rat 1:10 97.6
    Human 1:10 94.5
    Rabbit 1:10 103.5
    Dog 1:10 103.1
    Bovine fetus 1:10 105.2
    cell culture medium
    (10% bovine fetus serum added)
    RPMI 1:10 99.5
    DMEM 1:10 103.7

    Each serum and cell culture mediam (RPMI and DMEM with 10% bovine fetus serum) were used as samples.
    It was shown that dilution of each sample by 10 times or more was sufficient to measure OVA accurately.

  • ■ Reference

    Granzyme A Stimulates pDCs to Promote Adaptive Immunity via Induction of Type I IFN. Front. Immunol. 10, 1450 (2019). [Pubmed, Fulltext]

    Granzyme A Stimulates pDCs to Promote Adaptive Immunity via Induction of Type I IFN. Front. Immunol. 10, 1450 (2019). [Pubmed, Fulltext]

    Stable Immune Response Induced by Intradermal DNA Vaccination by a Novel Needleless Pyro-Drive Jet Injector. AAPS PharmSciTech 21, 19 (2019). [Pubmed, Fulltext]

    Systemic DC activation modulates the tumor microenvironment and shapes the long-lived tumor-specific memory mediated by CD8+ T cells. Cancer Res. 76, 3756–3766 (2016).[Pubmed]

    Topical skin treatment with Fab fragments of an allergen-specific IgG1 monoclonal antibody suppresses allergen-induced atopic dermatitis-like skin lesions in mice. Eur. J. Pharmacol. 779, 131–137 (2016). [Pubmed]

    Antigen exposure in the late light period induces severe symptoms of food allergy in an OVA-allergic mouse model. Sci. Rep. 5, 1–8 (2015). [Pubmed, Fulltext]

    Vaccination with antigen-transfected, NKT cell ligand-loaded, human cells elicits robust in situ immune responses by dendritic cells. Cancer Res. 73, 62–73 (2013). [PubMed, Fulltext]

    <Literature on this kit overview>
    Development of an enzyme-linked immunosorbent assay kit for measuring ovalbumin concentration in biomaterials.
    The Allergy in practice. 2012; 32: 177-180.

Note

All products that ITEA sells are reagent for experiment.
Cannot be used for treatment and diagnosis purposes.
We do not sell our products to individuals.